Search for biomarkers of animal exposure to cadmium

Journal Article · Wed Oct 01 04:00:00 EDT 1997 · Environmental and Molecular Mutagenesis

OSTI ID:530842

Ikediobi, C; Liu, Jing; Ugochukwu, Ngozi; Latinwo, L ^[1]

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1. Florida A&M Univ., Tallahassee, FL (United States)

Exposure of animals to cadmium has been associated with DNA strand breaks, and inactivation of sulfhydryl-containing enzymes and proteins. This study was intended to exploit certain Cd-induced cellular metabolic alterations/lesions with a view to develop reliable biomarkers of animal exposure to cadmium. Groups of Sprague-Dawley rats (125-150 g) were exposed by gavage on a daily basis to three different dose levels of CdCl{sub 2} (4.8, 12.0, 30.0 mg/kg) for periods of 3, 6, 9 and 12 days. At the end of each exposure period, blood, liver and kidney samples were obtained and analyzed for blood pyruvate, plasma trypsin inhibitory capacity (TIC) and liver/kidney malondialdehyde (MDA), metallothionein (MT) and Cd. There was a general decrease in bodyweight gain among the experimental groups of animals that was directly related to Cd dose. In addition, Cd in blood pyruvate and liver/kidney MDA were observed. Liver/kidney MT and total Cd were similarly observed to increase with Cd dose and duration of animal exposure to Cd. In contrast, plasma TIC decreased in a dose-dependent manner with increase in the duration of exposure. The relevance and significance of the use of blood pyruvate, liver/kidney MDA, MT, Cd and plasma TIC as potential biomarkers of animal exposure to Cd are discussed.

• OSTI ID: 530842
• Report Number(s): CONF-9704100--; CNN: Grant U50/ATU398948-04
• Journal Information: Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: Suppl.28 Vol. 29; ISSN 0893-6692; ISSN EMMUEG
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL INDICATORS
CADMIUM
CHRONIC EXPOSURE
RATS