Toxicity of corexit 9527 and Nigerian crude oil in vivo and in vitro
Journal Article
·
· Environmental and Molecular Mutagenesis
OSTI ID:530836
- Environmental Protection Agency, Research Triangle Park, NC (United States); and others
When oil is spilled into aquatic systems, chemical dispersants frequently are applied to enhance emulsification and biological availability. In this study, toxicity of Corexit 9527 (Cx), a widely used dispersant, was evaluated with oil both in vivo and in vitro. Initially, both Cx and oil were bioassayed in a micro-suspension modification of the Salmonella reversion bioassay. Next, rats were dosed 5 weeks with oil, Cx or Cx/oil. Body and tissue weights, urine mutagenicity, and effect on intestinal enzymes and microbiota were determined. Cx (1:1000 dilution) was toxic in the bioassay and oil was negative (TA98 & TA100 +/-S9). Similar results were observed for urinary metabolites. Body and tissue weights were unaffected, but a significant reduction in small intestinal azo reductase (AR) and/or {beta}-glucuronidase (BG) was observed in animals that received Cx, oil, or Cx/oil. Effects on cecal AR were observed in the 3 treatment groups. One week nitroreductase (NR) activity was elevated, and 3 and 5 week BG activities were significantly different from controls. This correspond to an elimination of enterobacteria and enterococci in oil-treated rats and enterobacteria fluctuations in Cx- and Cx/oil-treated animals. Even though treatment of rats with Cx has no direct effect on the production of mutagenic urine metabolites from oil-treated animals, significant changes are observed in intestinal microbiota and their enzymes responsible for promutagen and procarcinogen activation.
- OSTI ID:
- 530836
- Report Number(s):
- CONF-9704100--
- Journal Information:
- Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: Suppl.28 Vol. 29; ISSN 0893-6692; ISSN EMMUEG
- Country of Publication:
- United States
- Language:
- English
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