Effect of pentachlorophenol on the activation of 2,6-dinitrotoluene to genotoxic urinary metabolites in CD-1 mice: A comparison of G1 enzyme activities and urine mutagenicity

Journal Article · Mon Dec 31 23:00:00 EST 1990 · Environmental and Molecular Mutagenesis; (United States)

DOI:https://doi.org/10.1002/em.2850180203· OSTI ID:6093868

George, S E; Chadwick, R W; Creason, J P; Kohan, M J ^[1]; Dekker, J P ^[2]

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1. US Environmental Protection Agency, Research Triangle Park, NC (United States)
2. Environmental Health Research and Testing, Inc., Research Triangle Park, NC (United States)

2,6-Dinitrotoluene (2,6-DNT) and pentachlorophenol (PCP) are used for industrial purposes and are found in the environment as hazardous contaminants. Because concurrent exposure to both compounds can occur, it is of interest to determine if organochlorine compounds potentiate the effect of nitroaromatic chemicals. A significant increase in mutagenicity was observed in urines from mice treated with 2,6-DNT alone and in combination with PCP. By week 4, mice that received both 2,6-DNT and PCP excreted urine that was more mutagenic than that from animals which received only 2,6-DNT. At weeks 2 and 4, mice were sacrificed and intestinal enzyme activities (nitroreductase, azo reductase, {beta}-glucuronidase, dechlorinase, and dehydrochlorinase) were quantitated. The enhanced genotoxicity observed in urines from 2,6-DNT/PCP-treated mice coincided with a decrease in nitroreductase and an increase in {beta}-glucuronidase activities in the small intestine.

• OSTI ID: 6093868
• Journal Information: Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 18:2; ISSN 0893-6692; ISSN EMMUE
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATED AROMATICS
AROMATICS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHEMICAL ACTIVATION
CHLORINATED AROMATIC HYDROCARBONS
DIGESTIVE SYSTEM
ENZYME ACTIVITY
GASTROINTESTINAL TRACT
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
HYDROXY COMPOUNDS
MATERIALS
METABOLITES
NITRO COMPOUNDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
POLYCYCLIC NITRO COMPOUNDS
TOLUENE
URINE
WASTES