A 1.7-megabase sequence-ready cosmid contig covering the TSC1 candidate region in 9q34

Journal Article · Thu May 01 00:00:00 EDT 1997 · Genomics

DOI:https://doi.org/10.1006/geno.1997.4681· OSTI ID:530734

Hornigold, N; Wolfe, J; Nahmias, J ^[1]

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1. Univ. College London (United Kingdom); and others

The disease gene TSC1 has been genetically mapped to human chromosome region 9q34, in a 4-cM interval between the markers D9S149 and D9S114. Within this interval there is conflicting genetic evidence as to the finer localization of the gene. We have used finger-printing methods and hybridization to produce a 1.7-Mb overlapping clone map covering the TSC1. candidate region, with a single gap of 20 kb. We have localized 12 previously cloned genes and 17 genetic markers on this map and have confirmed the order of the genetic map. This deep set of overlapping clones is now ready to be used for candidate gene isolation, for transcription studies, or for sequencing. 27 refs., 3 figs.

• OSTI ID: 530734
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 41; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOLOGICAL MARKERS
CONTIGS
COSMIDS
DESIGN
DNA SEQUENCING
DNA-CLONING
DOMINANT MUTATIONS
ELECTROPHORESIS
FLUORESCENCE
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 9
IN-SITU HYBRIDIZATION
MENTAL DISORDERS
PATIENTS
PHENOTYPE
RESOLUTION
SKIN DISEASES
TRANSCRIPTION