Corneal endothelial glutathione after photodynamic change

Journal Article · Mon Mar 01 04:00:00 EST 1982 · Invest. Ophthalmol. Visual Sci.; (United States)

OSTI ID:5306889

Hull, D S; Riley, M V; Csukas, S; Green, K

Rabbit corneal endothelial cells perfused with 5 X 10(-6)M rose bengal and exposed to incandescent light demonstrated no alteration of either total of or percent oxidized glutathione after 1 hr. Addition of 5400 U/ml catalase to the perfusing solution had no effect on total glutathione levels but caused a marked reduction in percent oxidized glutathione in corneas exposed to light as well as in those not exposed to light. Substitution of sucrose for glucose in the perfusing solution had no effect on total or percent oxidized glutathione. Perfusion of rabbit corneal endothelium with 0.5 mM chlorpromazine and exposure to ultraviolet (UV) light resulted in no change in total glutathione content. A marked reduction in percent oxidized glutathione occurred, however, in corneas perfused with 0.5 mM chlorpromazine both in the presence and absence of UV light. It is concluded that photodynamically induced swelling of corneas is not the result of a failure of the glutathione redox system.

• Research Organization: Department of Ophthalmology, Medical College of Georgia, Augusta
• OSTI ID: 5306889
• Journal Information: Invest. Ophthalmol. Visual Sci.; (United States), Journal Name: Invest. Ophthalmol. Visual Sci.; (United States) Vol. 22:3; ISSN IOVSD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMALS
AZINES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
BODY AREAS
CELL CULTURES
CENTRAL NERVOUS SYSTEM AGENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMISTRY
CHLORPROMAZINE
COMPARATIVE EVALUATIONS
CORNEA
DRUGS
EDEMA
ELECTROMAGNETIC RADIATION
ENERGY SYSTEMS
EYES
FACE
FLUORESCENT LAMPS
GLUTATHIONE
HEAD
HETEROCYCLIC COMPOUNDS
HYPNOTICS AND SEDATIVES
LIGHT BULBS
LIGHTING SYSTEMS
MAMMALS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PEPTIDES
PHENOTHIAZINES
PHOTOSENSITIVITY
POLYPEPTIDES
PROTEINS
PSYCHOTROPIC DRUGS
RABBITS
RADIATIONS
RADIOPROTECTIVE SUBSTANCES
SENSE ORGANS
SENSITIVITY
SYMPTOMS
TRANQUILIZERS
ULTRAVIOLET RADIATION
VERTEBRATES
VISIBLE RADIATION