beta. -Adrenergic receptor-mediated suppression of interleukin 2 receptors in human lymphocytes
Adrenergic receptor agonists are know to attenuate the proliferative response of human lymphocytes after activation; however, their mechanism of action is unknown. Since expression of interleukin 2 (IL-2) receptors is a prerequisite for proliferation, the effect of ..beta..-adrenergic receptor agonists on lymphocyte IL-2 receptors was studied on both mitogen-stimulated lymphocytes and IL-2-dependent T lymphocyte cell lines. In both cell types the ..beta..-adrenergic receptor agonist isoproterenol blocked the expression of IL-2 receptors, as determined with the IL-2 receptor anti-TAC antibody. To determine the effect of ..beta..-adrenergic agonists on expression of the high affinity IL-2 receptors, (/sup 125/I)IL-2 binding studies were performed at concentrations selective for high affinity sites. No significant effect of ..beta..-adrenergic agonists on high affinity IL-2 receptor sites could be detected. The data demonstrate that ..beta..-adrenergic receptor agonists down-regulate IL-2 receptors primarily affecting low affinity sites.
- Research Organization:
- Univ. of Iowa College of Medicine, Iowa City
- OSTI ID:
- 5301564
- Journal Information:
- J. Immunol.; (United States), Vol. 139:10
- Country of Publication:
- United States
- Language:
- English
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