Proximal and distal regulatory elements that influence in vivo expression of a cell cycle-dependent human H4 histone gene

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.12.3982· OSTI ID:5301432

Kroeger, P; Steward, C; Schaap, T; van Wijnen, A; Hirshman, J; Helms, S; Stein, G; Stein, J

The authors have examined the sequences required in vivo to promote transcription of a cell cycle-regulated human H4 histone gene. Deletion mutants of the 5' flanking region were assayed in mouse cells or fused with the chloramphenicol acetyltransferase (CAT) gene for assay in HeLa cells. The functional limits of the regulatory sequences were shown to extend at least 6.5 kilobases (kb) upstream. Sequences sufficient for correctly initiated transcription were found in the 70 base pairs (bp) immediately 5' to the cap site. A proximal element located 200-400 bp upstream increased the level of transcription several times above the basal level, although not to maximal levels. Maximal levels of expression were achieved with 6.5 kb of 5' flanking sequence adjacent to the proximal promoter sequences or when a distal enhancer element with both position- and orientation-independent function was moved proximal to the promoter. The results indicate that a series of 5' cis-acting sequences are functionally related to the fidelity and level of expression of this human H4 histone gene.

• Research Organization: Univ. of Florida College of Medicine, Gainesville
• OSTI ID: 5301432
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Journal Issue: 12 Vol. 84:12; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ATP
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CYCLE
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
GENE REGULATION
GENES
HELA CELLS
HISTONES
IN VIVO
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MUTATIONS
NUCLEI
NUCLEOPROTEINS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VERTEBRATES