A photoactivable phospholipid analogue that specifically labels membrane cytoskeletal proteins of intact erythrocytes

Journal Article · Tue Aug 22 04:00:00 EDT 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00443a025· OSTI ID:5300479

Pradhan, D; Williamson, P; Schlegel, R A ^[1]

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1. Pennsylvania State Univ., University Park (USA)

A radioactive photoactivable analogue of phosphatidylethanolamine, 2-(2-azido-4-nitrobenzoyl)-1-acyl-sn-glycero-3-phospho({sup 14}C)ethanolamine(({sup 14}C)AzPE), was synthesized. Upon incubation with erythrocytes in the dark, about 90% of ({sup 14}C)AzPE spontaneously incorporated into the cells; of this fraction, about 90% associated with the membrane, all of it noncovalently. Upon photoactivation, 3-4% of the membrane-associated probe was incorporated into protein. Analysis of this fraction by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, as well as extraction of labeled membranes with alkali or detergent, showed that the probe preferentially labeled cytoskeletal proteins. ({sup 14}C)AzPE appears to be a useful tool for the study of lipid-protein interactions at the cytoplasmic face of the plasma membrane of intact cells.

• OSTI ID: 5300479
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 28:17; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
CHROMATOGRAPHY
CROSS-LINKING
DECOMPOSITION
ERYTHROCYTES
ESTERS
LABELLED COMPOUNDS
LABELLING
LIPIDS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
POLYMERIZATION
PROTEINS
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY