Determination of the distribution of selenium between selenoprotein P, glutathione peroxidase and albumin in plasma

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5300286

Whanger, P D; Butler, J A; Deagen, J T ^[1]

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1. Oregon State Univ., Corvallis (United States)

A chromatographic method was developed to determine the distribution of selenium between selenoprotein P, glutathione peroxidase (GSH-Px) and albumin, using two small columns of heparin-Sepharose CL-6B (white) and Reactive Blue 2-Sepharose CL-6B (blue) linked together in tandem. One ml of plasma was diluted to 10 ml with 0.02 M sodium phosphate buffer, pH 7.0, the equilibration buffer, and applied to the white column. This was eluted at flow rate of 30 ml per hour. GSHp-Px was not retained by either column but selenoprotein P was retained by the white column and albumin by the blue column. After the two columns were separated, selenoprotein P was eluted for 90 min from the white column with a solution containing 500 units of heparin per ml. The albumin was eluted for 55 min from the blue column with 1.4 M NaCl. This method indicated that over 50% of the selenium in plasma from rats, monkeys, humans and sheep was with selenoprotein P, even during deficiency. From 15 to 22% of the selenium was associated with GSH-Px. The percentage of selenium with albumin was dependent upon the selenomethionine content of the diet.

• OSTI ID: 5300286
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALBUMINS
ANIMALS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD PLASMA
BODY FLUIDS
DOMESTIC ANIMALS
DRUGS
ELEMENTS
ENZYMES
GLUTATHIONE
MAMMALS
MAN
MATERIALS
METABOLISM
METALLOPROTEINS
MONKEYS
NUTRITIONAL DEFICIENCY
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEPTIDES
PEROXIDASES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RATS
RODENTS
RUMINANTS
SELENIUM
SEMIMETALS
SHEEP
SYNTHESIS
VERTEBRATES