Determination of the distribution of selenium between selenoprotein P, glutathione peroxidase and albumin in plasma

Conference · Mon Mar 11 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5300286

Whanger, P D; Butler, J A; Deagen, J T ^[1]

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1. Oregon State Univ., Corvallis (United States)

A chromatographic method was developed to determine the distribution of selenium between selenoprotein P, glutathione peroxidase (GSH-Px) and albumin, using two small columns of heparin-Sepharose CL-6B (white) and Reactive Blue 2-Sepharose CL-6B (blue) linked together in tandem. One ml of plasma was diluted to 10 ml with 0.02 M sodium phosphate buffer, pH 7.0, the equilibration buffer, and applied to the white column. This was eluted at flow rate of 30 ml per hour. GSHp-Px was not retained by either column but selenoprotein P was retained by the white column and albumin by the blue column. After the two columns were separated, selenoprotein P was eluted for 90 min from the white column with a solution containing 500 units of heparin per ml. The albumin was eluted for 55 min from the blue column with 1.4 M NaCl. This method indicated that over 50% of the selenium in plasma from rats, monkeys, humans and sheep was with selenoprotein P, even during deficiency. From 15 to 22% of the selenium was associated with GSH-Px. The percentage of selenium with albumin was dependent upon the selenomethionine content of the diet.

• OSTI ID: 5300286
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
METALLOPROTEINS
BIOSYNTHESIS
SELENIUM
METABOLISM
ALBUMINS
BLOOD PLASMA
GLUTATHIONE
MAN
MONKEYS
NUTRITIONAL DEFICIENCY
PEROXIDASES
RATS
SHEEP
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
DOMESTIC ANIMALS
DRUGS
ELEMENTS
ENZYMES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RODENTS
RUMINANTS
SEMIMETALS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology