Mechanism of superinduction of the CYP1A1 gene by 2,3,7,8-tetrachlorodibenzo-P-dioxin (TCDD) plus cycloheximide
Conference
·
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
OSTI ID:5299863
- Stanford Univ., CA (United States)
TCDD increases CYP1A1 transcription by activating the Ah receptor, which binds to a specific DNA recognition sequence within an enhancer upstream of the CYP1A1 gene. Cycloheximide produces an additional increase of transcription. Nuclear runoff analyses of mouse hepatoma cells stably transfected with plasmids containing CYP1A1 regulatory DNA linked to a heterologous promoter and gene reveal that a DNA domain containing the receptor's recognition motif mediates superinduction. Gel retardation analyses reveal that mouse hepatoma cells contain a cycloheximide-sensitive, labile protein that binds to a DNA domain which overlaps the recognition motif for the Ah receptor. The labile protein is distinct from the Ah receptor in size and DNA binding specificity. Mutation of the binding site for the labile protein abolishes superinduction.
- OSTI ID:
- 5299863
- Report Number(s):
- CONF-9104107--
- Conference Information:
- Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
- Country of Publication:
- United States
- Language:
- English
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Thu Mar 14 23:00:00 EST 1991
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·
OSTI ID:5173553
Analysis of 2,3,7,8-tetrachlorodibenzo-p-dioxin responsive DNA sequences upstream of the cytochrome P sub 1 -450 gene
Thesis/Dissertation
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Thu Dec 31 23:00:00 EST 1987
·
OSTI ID:7243151
Antiestrogenic activity of 2,3,7,8-tetrachlorodibenzo-p-dioxin: Effect on expression of pS2, a prognostic marker for human breast cancer
Conference
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Sat Dec 30 23:00:00 EST 1995
·
OSTI ID:244867
Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CYCLOHEXIMIDE
DIOXIN
DISEASES
DNA
DRUGS
FUNGICIDES
GENES
GROWTH FACTORS
HEPATOMAS
HETEROCYCLIC COMPOUNDS
KINETICS
LYMPHOKINES
MAMMALS
MEMBRANE PROTEINS
MICE
MITOGENS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PESTICIDES
PLASMIDS
PROTEINS
REACTION KINETICS
RECEPTORS
RODENTS
TRANSCRIPTION
TUMOR CELLS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CYCLOHEXIMIDE
DIOXIN
DISEASES
DNA
DRUGS
FUNGICIDES
GENES
GROWTH FACTORS
HEPATOMAS
HETEROCYCLIC COMPOUNDS
KINETICS
LYMPHOKINES
MAMMALS
MEMBRANE PROTEINS
MICE
MITOGENS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PESTICIDES
PLASMIDS
PROTEINS
REACTION KINETICS
RECEPTORS
RODENTS
TRANSCRIPTION
TUMOR CELLS
VERTEBRATES