Sex pheromone receptor proteins. Visualization using a radiolabeled photoaffinity analog
A tritium-labeled photoaffinity analog of a moth pheromone was used to covalently modify pheromone-selective binding proteins in the antennal sensillum lymph and sensory dendritic membranes of the male silk moth, Antheraea polyphemus. This analog, (E,Z)-6,11-(/sup 3/H)hexadecadienyl diazoacetate, allowed visualization of a 15-kilodalton soluble protein and a 69-kilodalton membrane protein in fluorescence autoradiograms of electrophoretically separated antennal proteins. Covalent modification of these proteins was specifically reduced when incubation and UV irradiation were conducted in the presence of excess unlabeled pheromone, (E,Z)-6,11-hexadecadienyl acetate. These experiments constitute the first direct evidence for a membrane protein of a chemosensory neuron interacting in a specific fashion with a biologically relevant odorant.
- Research Organization:
- State Univ. of New York, Stony Brook (USA)
- OSTI ID:
- 5299371
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 263:8
- Country of Publication:
- United States
- Language:
- English
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MEMBRANE PROTEINS
CHEMICAL RADIATION EFFECTS
PHEROMONE
BIOCHEMICAL REACTION KINETICS
RECEPTORS
ACETATES
AUTORADIOGRAPHY
CELL MEMBRANES
COVALENCE
ELECTROPHORESIS
FLUORESCENCE
MOLECULAR WEIGHT
MOTHS
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
ANIMALS
ARTHROPODS
CARBOXYLIC ACID SALTS
CELL CONSTITUENTS
CHEMISTRY
ELECTROMAGNETIC RADIATION
INSECTS
INVERTEBRATES
KINETICS
LABELLED COMPOUNDS
LEPIDOPTERA
LUMINESCENCE
MEMBRANES
ORGANIC COMPOUNDS
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
SECRETION
550201* - Biochemistry- Tracer Techniques
560120 - Radiation Effects on Biochemicals
Cells
& Tissue Culture