Juvenile hormone receptors in insect larval epidermis: Identification by photoaffinity labeling

Journal Article · Mon Jan 01 04:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.2.796· OSTI ID:6699974

Palli, S R; Osir, E O; Edwards, M; Hiruma, K; Riddiford, L M ^[1]; Eng, W; Boehm, M F; Kulscar, P; Ujvary, I; Prestwich, G D ^[2]

---

1. Univ. of Washington, Seattle (USA)
2. State Univ. of New York, Stony Brook (USA)

Tritiated photoaffinity analogs of the natural lepidopteran juvenile hormones, JH I and II (epoxy({sup 3}H)bishomofarnesyl diazoacetate (({sup 3}H)EHDA) and epoxy({sup 3}H)homofarnesyl diazoacetate (({sup 3}H)EHDA)), and of the JH analog methoprene (({sup 3}H)methoprene diazoketone (({sup 3}H)MDK)) were synthesized and used to identify specific JH binding proteins in the larval epidermis of the tobacco hornworm (Manduca sexta). EBDA and EHDA specifically photolabeled a 29-kDa nuclear protein (pI 5.8). This protein and a second 29-kDa protein (pI 6.0) were labeled by MDK, but excess unlabeled methoprene or MDK only prevented binding to the latter. These 29-kDa proteins are also present in larval fat body but not in epidermis from either wandering stage or allatectomized larvae, which lack high-affinity JH binding sites. A 29-kDa nuclear protein with the same developmental specificity as this JH binder bound the DNA of two larval endocuticle genes. A 38-kDa cytosolic protein was also specifically photolabeled by these photoaffinity analogs. The 29-kDa nuclear protein is likely the high-affinity receptor for JH that mediates its genomic action, whereas the 38-kDa cytosolic protein may serve as an intracellular carrier for these highly lipophilic hormones and hormone analogs.

• OSTI ID: 6699974
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 87:2; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ANIMAL TISSUES
ANIMALS
ARTHROPODS
BIOCHEMISTRY
BIOSYNTHESIS
BODY
CARBOXYLIC ACID SALTS
CHEMISTRY
DIAZO COMPOUNDS
DISTRIBUTION
EPIDERMIS
EPITHELIUM
HORMONES
HYDROGEN COMPOUNDS
INSECTS
INVERTEBRATES
JUVENILES
LABELLING
LARVAE
LEPIDOPTERA
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
RECEPTORS
SKIN
SYNTHESIS
TISSUE DISTRIBUTION
TISSUES
TRITIUM COMPOUNDS