Accumulation of RNA homologous to human papillomavirus type 16 open reading frames in genital precancers

Journal Article · Thu Dec 31 23:00:00 EST 1987 · J. Virol.; (United States)

OSTI ID:5299248

Crum, C P; Nuovo, G; Friedman, D; Silverstein, S J

The accumulation of human papillomavirus type 16 (HPV-16)-specific RNAs in tissue sections from biopsies of patients with genital precancers was studied by in situ hybridization with single-stranded /sup 35/S-labeled RNA. These analyses revealed that the most abundant early-region RNAs were derived from the E4 and E5 open reading frames (ORFs). RNAs homologous to the E6/E7 ORFs were also detected, whereas RNAs homologous to the intervening E1 ORF were not. This suggest that the E4 and E5 mRNAs are derived by splicing to the upstream E6/E7 ORFs, consistent with studies of HPV-11 in condylomata. Abundant RNAs homologous to the 5' portion of L1 were also detected. These RNAs were localized to the apical strata of the epithelium. HPV-16 RNAs accumulated in discrete regions of these lesions, and when present were most abundant in the upper cell layers of the precancerous epithelium. RNAs homologous to early ORFs were also detected in some germinal cells within the basal layer of the epithelium.

• Research Organization: Columbia Univ., New York
• OSTI ID: 5299248
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 62:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CARCINOGENESIS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
EPITHELIUM
EVEN-ODD NUCLEI
HYBRIDIZATION
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MESSENGER-RNA
MICROORGANISMS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PATHOGENESIS
PATIENTS
RADIOISOTOPES
RNA
SULFUR 35
SULFUR ISOTOPES
TISSUES
VIRUSES