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Adsorption of proteins from artificial tear solutions to contact lens materials

Journal Article · · Invest. Ophthalmol. Visual Sci.; (United States)
OSTI ID:5299219
; ; ;
A series of polymers and copolymers of 2-hydroxyethyl methacrylate (HEMA) and methyl methacrylate (MMA) were synthesized in order to find surfaces that would adsorb minimal amounts of protein. The adsorption of albumin, lysozyme and immunoglobulin G from a three-way mixture of these proteins in isotonic buffered saline to the polymers was measured using /sup 125/I-labeled proteins. Apparently high protein uptake on copolymers rich in HEMA was found to be due to sorption of unbound /sup 125/I by the polymers. /sup 125/I sorption by the polymers was minimized by dialysis of the protein solution to remove unbound /sup 125/I iodide and inclusion of 0.01 M sodium iodide to block uptake of residual /sup 125/I iodide. Using these improved protocols, minimal total protein uptake was observed on copolymers containing 50% or more HEMA. The majority of adsorbed protein on all p(MMA-HEMA) polymers was albumin. Total protein uptake was greatest on pMMA. Commercial contact lenses composed of copolymers of HEMA and N-vinyl pyrrolidone (NVP) or acrylamide (AAm) adsorbed small amounts of all proteins whereas copolymers of methacrylic acid (MAAc) and HEMA adsorbed much larger quantities of lysozyme. These results indicate that protein uptake by contact lens materials varies greatly with polymer composition. Artifactually high adsorption can occur if precautions are not taken to prevent uptake of unbound /sup 125/I.
Research Organization:
Univ. of Washington, Seattle (USA)
OSTI ID:
5299219
Journal Information:
Invest. Ophthalmol. Visual Sci.; (United States), Journal Name: Invest. Ophthalmol. Visual Sci.; (United States) Vol. 29:3; ISSN IOVSD
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550600 -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ADSORPTION
ALBUMINS
BETA DECAY RADIOISOTOPES
BODY
BODY AREAS
CARBOXYLIC ACID SALTS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EYES
FACE
GLOBULINS
HEAD
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LYSOSOMES
MEDICINE
METHACRYLATES
NUCLEI
ODD-EVEN NUCLEI
OPHTHALMOLOGY
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
POLYMERS
PROTEINS
RADIOISOTOPES
SENSE ORGANS
SORPTION
TRACER TECHNIQUES