Comparison of effects of ATP-MgCl/sub 2/ and adenosine-MgCl/sub 2/ on renal function following ischemia

Journal Article · Sun Feb 01 00:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:5298913

Sumpio, B E; Hull, M J; Baue, A E; Chaudry, I H

ATO-MgCl/sub 2/ administration had been shown to accelerate the recovery of renal function following warm ischemia. However, since the major breakdown product of ATP is adenosine, the relative contribution of ATP vs. adenosine in improving renal function following ischemia remains to be determined. To study this, kidneys were subjected to 45 min of normothermic ischemia and then perfused at 100 mmHg with oxygenated Krebs-HCO/sub 3/ buffer containing albumin, (/sub 3/H)inulin, substrates, and either 0.3 mM ATP-MgCl/sub 2/ or adenosine-MgCl/sub 2/ for 110 min. Perfusate and timed urine samples were collected and analyzed for radioactivity and (Na/sup +/). The functional parameters indicated that although adenosine-MgCl/sub 2/ treatment provided a transient improvement, it failed to provided a sustained improvement in renal function or attain control valued compared with ATP-MgCl/sub 2/ treatment. Thus, the salutary effects of ATP-MgCl/sub 2/ following warm ischemia in the kidney are not mediated by adenosine.

• Research Organization: Yale Univ. School of Medicine, New Haven, CT
• OSTI ID: 5298913
• Journal Information: Am. J. Physiol.; (United States), Vol. 252:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
BIOCHEMISTRY
ATP
KIDNEYS
PHYSIOLOGY
TRITIUM COMPOUNDS
TRACER TECHNIQUES
INULIN
ISCHEMIA
MAGNESIUM CHLORIDES
URINE
ALKALINE EARTH METAL COMPOUNDS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
CARBOHYDRATES
CARDIOVASCULAR DISEASES
CHEMISTRY
CHLORIDES
CHLORINE COMPOUNDS
DISEASES
HALIDES
HALOGEN COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAGNESIUM COMPOUNDS
MATERIALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
RIBOSIDES
SACCHARIDES
VASCULAR DISEASES
WASTES
551001* - Physiological Systems- Tracer Techniques