Rabbit kidney releases 6-keto-prostaglandins E/sub 1/ (6-K-PGE/sub 1/)

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5326275

Pieroni, J P; Dray, F; Pace-Asciak, C; McGiff, J C

The authors studied the production of radioimmunoassayable (i-) 6-K-PGE/sub 1/ - a potent renin secretagogue, vasodilator and platelet anti-aggregatory agent - in the rabbit isolated kidney perfused at 2 ml/g/min with a modified Krebs'-Henseleit solution (37/sup 0/C). The ureter was cannulated, enabling separate collections or urinary and venous effluents. 6-K-PGE/sub 1/ levels were determined by RIA after acidic lipid extraction and high performance liquid chromatographic (HPLC) separation. The authors used arachidonic acid (AA) and adenosine triphosphate (ATP) to release 1-6-K-PGE/sub 1/ as they had stimulated the greatest release of prostacyclin as measured by i-6-K-PGR/sub 1..cap alpha../. When either AA or ATP was given into the renal artery, i-6-K-PGE/sub 1/ was released into both urinary and venous effluents in pg/ml concentrations. More i-6-K-PGE/sub 1/ (3 to 10 times) was found in the urinary than in the venous effluent. HPLC zones co-eluting with authentic 6-K-PGE/sub 1/ and yielding i-6-K-PGE/sub 1/ possessed potent platelet anti-aggregatory activity, while adjacent zones did not. 6-K-PGE/sub 1/ was also detected by GC-MS (single ion monitoring), approximately 4 ng in pooled effluents from 11 kidneys. Thus, the authors have recovered immunoreactive and biologically active material indistinguishable from 6-K-PGE/sub 1/, evoked by AA and ATP, in both urinary and venous effluents of the rabbit kidney. The authors have also recovered i-6-K-PGE/sub 1/ from incubates of rabbit renal cortical slices with PGE/sub 2/ added.

• Research Organization: New York Medical College, Valhalla
• OSTI ID: 5326275
• Report Number(s): CONF-8604222-; TRN: 86-030440
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
ATP
PROSTAGLANDINS
BIOSYNTHESIS
RADIOIMMUNOASSAY
GAS CHROMATOGRAPHY
IMMUNE REACTIONS
KIDNEYS
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
PERFUSED ORGANS
RABBITS
ANIMALS
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MONOCARBOXYLIC ACIDS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOASSAY
RADIOIMMUNOLOGY
SEPARATION PROCESSES
SPECTROSCOPY
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques