Neurosecretory effect of ouabain on isolated rabbit ileal mucosa
Ouabain, when added to fluid bathing rabbit ileal mucosa mounted in a flux chamber, transiently increases short circuit current, implying a paradoxical secretory response. To determine the cause of this change, the authors studied unidirectional fluxes of /sup 36/Cl and /sup 23/Na and the effects of ion substitution, of reduced Ca concentration, verapamil, tetrodotoxin and atropine. Ouabain 0.1 mM, transiently increased the serosal to mucosal flux of Cl and Na, increased Isc and PD and reduced ion conductance. The Isc response to ouabain was diminished by reducing the bath fluid concentration of CL, of Ca, and by adding verapamil. Tetrodotoxin both delayed and reduced the maximal Isc response; atropine had no effect. They conclude that ouabain acts by releasing a neurotransmitter of unknown identity and by increasing the serosal to mucosal flux to Cl.
- Research Organization:
- Univ. of Iowa, Iowa City (USA)
- OSTI ID:
- 5298839
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 42:18; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL ISOTOPES
ALKALINE EARTH METALS
ALKALOIDS
ANIMALS
ANTIGENS
ATROPINE
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOELECTRICITY
BIOLOGICAL EFFECTS
BODY
CALCIUM
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHLORINE
CHLORINE 36
CHLORINE ISOTOPES
DIGESTIVE SYSTEM
DRUGS
ELECTRICITY
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
GASTROINTESTINAL TRACT
GLYCOSIDES
HALOGENS
INTESTINES
ION EXCHANGE
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
MEMBRANES
METALS
MUCOUS MEMBRANES
NONMETALS
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OUABAIN
PARASYMPATHOLYTICS
RABBITS
RADIOISOTOPES
RESPONSE MODIFYING FACTORS
SMALL INTESTINE
SODIUM 23
SODIUM ISOTOPES
STABLE ISOTOPES
STROPHANTHINS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VASODILATORS
VERTEBRATES
YEARS LIVING RADIOISOTOPES