Site and mechanisms of action of kinins in rat ileal mucosa

Journal Article · Sun Mar 01 04:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:5356357

Warhurst, G; Lees, M; Higgs, N B; Turnberg, L A

Kinin-induced secretion in the intestine is accompanied by marked increases in mucosal adenosine 3',5'-cyclic monophosphate (cAMP) and prostanoids that undoubtedly contribute to the overall secretory responses. The authors have investigated the effects of kallidin on the phospholipase-prostanoid-cAMP pathway in whole ileal mucosa and in epithelial cells isolated from the same tissue in the rat. Kallidin (1 ..mu..M) stimulated a marked rise in PG (prostaglandin) E/sub 2/ release from the serosal surface of stripped ileal mucosa within 1-2 min, which correlated closely with the rise in mucosal short-circuit current. Mucosal cAMP levels were also increased two to threefold by kallidin. However, kinins were unable to elicit effects under the same conditions in suspensions of viable epithelial cells. PGE/sub 2/ release was unaffected by kallidin or bradykinin at concentrations up to 100 ..mu..M, whereas cAMP levels could be stimulated by forskolin and PGE/sub 2/ but not by kinin. Studies of intestinal phospholipase A/sub 2/ (PLA/sub 2/) activity also suggest a nonepithelial site for kinin action. In the intestine, PLA/sub 2/ activity was found to be concentrated within the subepithelium with significantly lower levels in the epithelium itself. In addition, kallidin was unable to influence phospholipid labeling (an indirect measure of PLA/sub 2/ activity) in cells incubated with (/sup 14/C) arachidonic acid. These studies suggest that kinins initiate increases in intestinal prostaglandin and cAMP production within the subepithelium and not by a direct action on epithelial cells.

• Research Organization: Univ. of Manchester School of Medicine, Salford, England
• OSTI ID: 5356357
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 252:3; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMP
ANIMAL TISSUES
ANIMALS
ARACHIDONIC ACID
BIOCHEMISTRY
BODY
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CHEMISTRY
DIGESTIVE SYSTEM
ENZYMES
EPITHELIUM
ESTERASES
ESTERS
GASTROINTESTINAL TRACT
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
INTESTINES
ISOTOPE APPLICATIONS
KININS
LABELLED COMPOUNDS
LIPASE
LIPIDS
MAMMALS
MEMBRANES
MONOCARBOXYLIC ACIDS
MUCOUS MEMBRANES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PEPTIDES
PHOSPHOLIPIDS
POLYPEPTIDES
PROSTAGLANDINS
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
RODENTS
SECRETION
TISSUES
TRACER TECHNIQUES
VERTEBRATES