Comparison of (/sup 14/C)glucose and (/sup 14/C)deoxyglucose as tracers of brain glucose use

Journal Article · Tue Mar 01 00:00:00 EST 1988 · Am. J. Physiol.; (United States)

OSTI ID:5295866

Hawkins, R A; Mans, A M; Davis, D W; DeJoseph, M R

Because glucose metabolism and functional activity in brain regions are normally coupled, knowledge of regional brain glucose use can yield insights into regional functional activity. The deoxyglucose (DG) method is widely used for this purpose in experimental animals and humans but questions have arisen regarding its limits and accuracy. Therefore an experiment was designed to compare the DG method on a structure-by-structure basis with another tracer of glucose use, (6-/sup 14/C)glucose, in normal rats. The cerebral metabolic rates obtained using the two tracers were similar in the telencephalon, but the results using DG were substantially lower in the midbrain and hindbrain (diencephalon, 18%; mesencephalon, 20%; metencephalon, 29%; and myelencephalon, 35%). The primary DG metabolite, DG 6-phosphate (DG-6-P) was found to disappear in a non-uniform manner from the major brain structures: telencephalon less than diencephalon less than mesencephalon = metencephalon less than myelencephalon. Thus a correlation was found between the rate of DG-6-P loss and the extent to which the DG method gave lower values of glucose use. Thus this may explain, at least in part, the discrepancies between the two methods.

• Research Organization: Hershey Medical Center, PA (USA)
• OSTI ID: 5295866
• Journal Information: Am. J. Physiol.; (United States), Vol. 254
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSE
METABOLISM
AUTORADIOGRAPHY
BRAIN
CARBON 14 COMPOUNDS
COMPARATIVE EVALUATIONS
METABOLITES
RATS
ALDEHYDES
ANIMALS
BODY
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM
HEXOSES
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
RODENTS
SACCHARIDES
VERTEBRATES
550501* - Metabolism- Tracer Techniques