Reaction of copper-zinc superoxide dismutase with diethyldithiocarbamate

Journal Article · Sun Nov 25 04:00:00 EST 1979 · J. Biol. Chem.; (United States)

OSTI ID:5292794

Misra, H P

Diethyldithiocarbamate reacted with superoxide dismutase from bovine erythrocytes. Changes in both optical and esr spectra, which accompanied this reaction, indicated involvement of the Cu(II). The reaction was accelerated by raising the concentrations of the reactants, elevating the temperature, and lowering the pH, in the range 10.2 to 5.5, and it was independent of the presence of oxygen. During the first phase of this reaction the Cu(II).diethyldithiocarbamate complex remained bound to the enzyme and the catalytic activity did not diminish. There followed a second and slower process which was accompanied by the appearance of colloidal Cu(II).chelate complex and by a loss of activity which could be restored by the addition of CuSO/sub 4/. All of the observations are accommodated by a model in which 1 diethyldithiocarbamate molecule reacts/copper center, with retention of activity, in Phase I, while a second diethyldithiocarbamate displaces the copper, with a loss of activity, in Phase II.

• Research Organization: Univ. of California, Davis
• OSTI ID: 5292794
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 254:22; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBAMATES
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CATALYSIS
COMPLEXES
COPPER
ELEMENTS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYME REACTIVATION
ENZYMES
ERYTHROCYTES
KINETICS
MATERIALS
METALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PH VALUE
RADICALS
REACTION KINETICS
SUPEROXIDE RADICALS
TEMPERATURE EFFECTS
TRANSITION ELEMENTS