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Peroxisomal oxidation of very long chain fatty acids (VLCFA) by human hepatoma cells

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5277157
;

Beta-oxidation of VLCFA was studied in a human hepatoma cell line (HEP-G2). These cells, disrupted by exposure to low concentrations of digitonin, oxidize (1-/sup 14/C)palmitate (C16:0) and (1-/sup 14/C)lignocerate (C24:0) to /sup 14/CO/sub 2/ and water-soluble products. It was recently reported that in rat liver the beta-oxidation of VLCFA takes place primarily in the peroxisome rather than the mitochondrion. The precise site of VLCFA oxidation in human tissues has not been clearly elucidated. The peroxisome has been implicated since there is impaired VLCFA oxidation in fibroblasts from Zellweger syndrome patients, in which this organelle is deficient. In order to define the subcellular localization of human VLCFA oxidation, homogenates of HEP-G2 cells were fractionated on a discontinuous sucrose gradient. Fractions enriched in the peroxisomal marker catalase oxidized C24:0 at significantly greater rates than fractions enriched in the mitochondrial marker succinate:cytochrome c reductase. C16:0 oxidation was catalyzed by both peroxisomal and mitochondrial fractions. These results suggest that the subcellular site of VLCFA oxidation in human hepatoma cells and rat liver is similar.

Research Organization:
Kennedy Institute, Baltimore, MD
OSTI ID:
5277157
Report Number(s):
CONF-8606151-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:6; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL LOCALIZATION
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
DISEASES
FIBROBLASTS
GLANDS
HEPATOMAS
HEXADECANOIC ACID
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
MAN
METABOLISM
MONOCARBOXYLIC ACIDS
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PATIENTS
PRIMATES
RATS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES