Increased concentrations of lysophosphatidylcholine in cardiac lymph from ischemic zones

Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5277047

Corr, P B; Akita, H; Creer, M H; Yamada, K A; Sobel, B E

The authors have previously shown that lysophosphatidylcholine (LPC) elicits electrophysiological derangements in normoxic cells exposed to low concentrations of LPC with or without albumin in the extracellular fluid. To determine whether such exposure occurs in vivo, concentrations of phospholipids in cardiac lymph were assessed in 7 dogs subjected to myocardial ischemia. In each animal, a cannula was inserted into the supracardiac lymph vessel identified with Evans Blue dye injected into the left ventricular apex. Less than 4% of /sup 14/C-PC (phosphatidyl choline) or /sup 14/C-LPC incubated in cardiac lymph for 30 min at 4/sup 0/C or 90 min at 37/sup 0/C was catabolized (n = 5). Lymph was collected on ice at 15 min intervals throughout 2 hr of ischemia induced by LAD coronary ligation, extracted in CHCl/sub 3/:CH/sub 3/OH (2:1) and phospholipids separated by HPLC. Prior to ischemia, PC constituted the major phospholipid (79 +/- 2%). Substantial quantities of sphinogomeylin (11 +/- 2%) and LPC (6 +/- 1%) were present. With ischemia, LPC concentration increased 134% from 84 +/- 5 to 197 +/- 34 ..mu..M (n = 7, p < .01) judging from the measured concentration and dilution determined with constant infusion of dye into the LV apex. In contrast, PC concentration and efflux did not change. Thus with ischemia LPC increases in interstitial fluid to levels sufficient to induce electrophysiologic derangements in normoxic cells.

• Research Organization: Washington Univ., St. Louis, MO
• OSTI ID: 5277047
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
ANIMALS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBON 14 COMPOUNDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CHOLINE
CHROMATOGRAPHY
DISEASES
DISTRIBUTION
DOGS
DRUGS
ELECTROPHYSIOLOGY
ESTERS
HEART
HYDROXY COMPOUNDS
IN VIVO
ISCHEMIA
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
LYMPH
MAMMALS
MATERIALS
MUSCLES
MYOCARDIUM
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PATHOGENESIS
PHOSPHOLIPIDS
PHYSIOLOGY
QUATERNARY COMPOUNDS
SEPARATION PROCESSES
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES