Purification and partial characterization of a. beta. -glucan fragment that elicits phytoalexin accumulation in soybean

Journal Article · Tue Sep 25 00:00:00 EDT 1984 · J. Biol. Chem.; (United States)

OSTI ID:5273064

Sharp, J K; Valent, B; Albersheim, P

The synthesis of phytoalexins (antibiotics) in plant cells is induced by molecules called elicitors. Partial acid hydrolysis of mycelial walls of Phytophthora megasperma f. sp. glycinea solubilized a multicomponent mixture of elicitor-active and elicitor-inactive oligoglucosides. One elicitor-active and seven elicitor-inactive hexa(..beta..-D-glucopyranosyl)-D-glucitols were purified by gel-filtration, normal-phase partition, and reversed-phase liquid chromatography after reduction with NaBH/sub 4/. The elicitor-active and all but one of the elicitor-inactive hexa(..beta..-D-glucopyranosyl)-D-glucitols consisted of 3-, 6-, and 3,6-linked glucopyranosyl residues. The eighth hexa(..beta..-D-glucopyranosyl)-D-glucitol consisted of ..beta..-4-linked glucopyranosyl residues. The similarity of the structural characteristics of six of the elicitor-inactive hexa(..beta..-D-glucopyranosyl)-D-glucitols to the elicitor-active hexa(..beta..-D-glucopyranosyl)-D-glucitols suggested that a highly specific carbohydrate structure was required for elicitor activity. 47 references, 7 figures, 4 tables.

• Research Organization: Univ. of Colorado, Boulder
• DOE Contract Number: AC02-76ER01426
• OSTI ID: 5273064
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 259:18; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BACTERIA
BIOLOGICAL PATHWAYS
BIOLOGY
BIOSYNTHESIS
CARBOHYDRATES
DRUGS
ENZYME ACTIVITY
FUNGI
GENETICS
GLYCINE HISPIDA
LEGUMINOSAE
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
RHIZOBIUM
SACCHARIDES
STRUCTURE-ACTIVITY RELATIONSHIPS
SYNTHESIS