Hypoxic fraction and binding of misonidazole in EMT6/Ed multicellular tumor spheroids
Misonidazole has been shown to bind selectively to hypoxic cells in tissue culture and to cells which are presumed to be chronically hypoxic in EMT6 spheroids and tumors. Thus it has considerable potential as a marker of hypoxic cells in vivo. To further evaluate this potential EMT6/Ed spheroids were used to quantitate misonidazole binding under conditions which resulted in hypoxic fractions between 0 and 1. The patterns of binding of /sup 14/C-labeled misonidazole determined by autoradiography were consistent with the regions of radiobiological hypoxia as predicted by oxygen diffusion theory. The overall uptake of /sup 3/H-labeled misonidazole by spheroids correlated well with the hypoxic fraction, although binding to aerobic cells and necrotic tissue contributed appreciably to the total label in the spheroids. It is concluded that misonidazole is an excellent marker of hypoxia in EMT6/Ed spheroids at the microscopic level, and the total amount bound per spheroid provides a potentially useful measure of the hypoxic fraction.
- Research Organization:
- Univ. of Alberta, Edmonton
- OSTI ID:
- 5265446
- Report Number(s):
- CONF-860423-
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 103:1; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANOXIA
ANTINEOPLASTIC DRUGS
AUTORADIOGRAPHY
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBON 14 COMPOUNDS
CESIUM 137
CESIUM ISOTOPES
DRUGS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
IONIZING RADIATIONS
ISOTOPES
LABELLED COMPOUNDS
MISONIDAZOLE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RADIOSENSITIZERS
SPHEROIDS
SURVIVAL CURVES
TRITIUM COMPOUNDS
TUMOR CELLS
UPTAKE
YEARS LIVING RADIOISOTOPES