Preferential DNA repair of 3-alkyladenine sites in essential and nonessential genes of human astrocytes

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5264036

Lapcevich, R K; Weiss, R B; Gallagher, P E ^[1]

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1. West Virginia Univ., Morgantown (United States)

In recent years, numbers of studies examining excision rates of DNA damaged lesions in defined, subgenomic sequences have shown that DNA repair is not a uniform process throughout the genome. Here, the authors report data on the preferential, in vivo DNA repair of alkylation-induced lesions within specific DNA sequences of essential and nonessential genes. The formation and rate of removal of 3-alkyladenine were studied in these DNA fragments following treatment of human astrocytes with dimethyl sulfate. The distribution and quantitation of this damaged lesion in the isolated DNA from these cells were determined by a polymerase chain reaction assay. The results indicate that alkyladenines are more efficiently repaired in DNA fragments of essential genes than in comparable fragments of nonessential genes. In subsequent experiments, the repair rate of 3-alkyladenine was examined in DNA isolated from alkylation-treated human astrocytes, grown in serum-free medium to inhibit proliferation. The rate of repair of alkylation-induced lesions in essential and nonessential gene fragments also differed in actively growing and quiescent human astrocytes. The results of this study indicate that transcription plays an important role in the efficient removal of 3-alkyladenine by DNA repair systems.

• OSTI ID: 5264036
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENINES
ALKYLATING AGENTS
ALKYLATION
AMINES
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AROMATICS
AZAARENES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL REACTIONS
DNA REPAIR
DRUGS
ENZYMES
GENES
GENETIC EFFECTS
HETEROCYCLIC COMPOUNDS
MAMMALS
MAN
NERVE CELLS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
PROTEINS
PURINES
REPAIR
SOMATIC CELLS
TRANSCRIPTION
TRANSFERASES
VERTEBRATES