The RAD6 gene and protein of Saccharomyces cerevisiae

Conference · Sat Dec 31 04:00:00 EST 1994

OSTI ID:135569

Prakash, L ^[1]

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1. Univ. of Texas Medical Branch, Galveston, TX (United States)

Repair mechanisms play an essential role in maintaining the integrity of the structure and function of DNA, and it is therefore not surprising that a significant proportion of the genome functions in the surveillance of DNA and that a variety of mechanisms have evolved to repair damage to DNA. Ultraviolet light causes the formation of two main types of DNA lesions: cyclobutane pyrimidine dimers and pyrimidine (6-4) pyrimidone photoproducts. This results in local helix distortion, and such a template is a poor substrate for transcription and DNA replication. These lesions are removed from DNA by an excision repair mechanism. In Escherichia coli, the products of the UvrABC genes are involved in incision of the damaged DNA strand on the 5{prime} and 3{prime} sides of the dimer. The fragment is then removed and the gap filled in by resynthesis using the opposite strand as a template, followed by ligation.

• Research Organization: New York Academy of Sciences, New York, NY (United States)
• OSTI ID: 135569
• Report Number(s): CONF-9307221--; CNN: Grant GM19261
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL MODELS
COMPARATIVE EVALUATIONS
CONFORMATIONAL CHANGES
DNA
DNA HELICASES
DNA REPAIR
DNA REPLICATION
ENZYMES
GENES
GENETIC RADIATION EFFECTS
MUTATIONS
PROTEINS
PYRIMIDINE DIMERS
SACCHAROMYCES CEREVISIAE
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSCRIPTION
ULTRAVIOLET RADIATION