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Deoxyribonucleic acid repair in Bacillus subtilis: development of competent cells into a tester for carcinogens

Journal Article · · Appl. Environ. Microbiol.; (United States)
OSTI ID:5263083
;
The development of competent transformed Bacillus subtilis into a tester system for carcinogens is described. Precocious or noninduced activation of SOS functions occurs in competent cells. Thus, lower doses or concentrations of SOS inducing agents are needed to cause cell death due to indigenous prophage activation and lysis of bacteria. The two known defective prophages in B. subtilis enhance the sensitivity of competent cells to the carcinogens ultraviolet light, mitomycin C, and methyl methanesulfonate. However, these same cells have no enhanced sensitivity for the non-carcinogenic ethyl methanesulfonate or for nalidixic acid. Therefore, competent B. subtilis appears to be a sensitive tester for carcinogens.
Research Organization:
Pennsylvania State Univ., University Park
OSTI ID:
5263083
Journal Information:
Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 39:4; ISSN AEMID
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
550700 -- Microbiology
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIBIOTICS
ANTIMITOTIC DRUGS
BACILLUS
BACILLUS SUBTILIS
BACTERIA
BIOASSAY
BIOLOGICAL INDICATORS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARCINOGENESIS
CARCINOGENS
CELL KILLING
DNA
DRUGS
ELECTROMAGNETIC RADIATION
ESTERS
METHYL METHANESULFONATE
MICROORGANISMS
MITOMYCIN
MUTAGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PATHOGENESIS
RADIATIONS
RECOVERY
REPAIR
SULFONIC ACID ESTERS
TOXICITY
ULTRAVIOLET RADIATION