Rabbit hindlimb glucose uptake assessed with positron-emitting fluorodeoxyglucose

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · Journal of Applied Physiology (1985); (USA)

OSTI ID:5262830

Mossberg, K A; Rowe, R W; Tewson, T J; Taegtmeyer, H ^[1]

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1. Univ. of Texas Health Science Center, Houston (USA)

The feasibility of estimating skeletal muscle glucose uptake in vivo was examined by using the glucose analogue 2-({sup 18}F)deoxy-2-fluoro-D-glucose (2-({sup 18}F)FDG) in the rabbit hindlimb. A pair of collimated coincidence gamma photon detectors was used to monitor the accumulation of tracer in the tissue after 2-({sup 18}F)FDG injection. Time-activity curves were generated on a second-by-second basis under control conditions, during increased contractile activity, or hyperinsulinemia. The arterial input of 2-({sup 18}F)FDG, plasma glucose, lactate, free fatty acids, and insulin were determined. A graphical (Patlak plot) procedure was used to determine the fractional rate of tracer phosphorylation and therefore trapping in the muscle. From the graphical analysis, the estimated rate of glucose phosphorylation (R) in the unperturbed state was calculated to be 0.037 mumol.min-1.ml-1 of tissue. During perturbation by electrical stimulation, an increase in the rate of tracer phosphorylation (K) was observed. No change in the rate of tracer phosphorylation was observed during hyperinsulinemia. The results support the use of 2-({sup 18}F)FDG and the graphical procedure for the noninvasive assessment of glucose uptake by skeletal muscle in vivo. The method described is sensitive to changes in the rate of tracer uptake with respect to time and physiological interventions.

• OSTI ID: 5262830
• Journal Information: Journal of Applied Physiology (1985); (USA), Journal Name: Journal of Applied Physiology (1985); (USA) Vol. 67:4; ISSN 8750-7587; ISSN JAPHE
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
BODY AREAS
CARBOHYDRATES
CHEMICAL REACTIONS
CONTRACTION
ELECTRIC SHOCK
FLUORINE 18
FLUORINE ISOTOPES
GLUCOSE
HEXOSES
HORMONES
HOURS LIVING RADIOISOTOPES
INSULIN
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEGS
LIGHT NUCLEI
LIMBS
MAMMALS
MEASURING METHODS
METABOLISM
MONOSACCHARIDES
MUSCLES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PHOSPHORYLATION
RABBITS
RADIOISOTOPES
SACCHARIDES
TRACER TECHNIQUES
UPTAKE
VERTEBRATES