Calculation and experimental verification of solute retention in liquid chromatography using binary eluents

Journal Article · Mon Jun 01 04:00:00 EDT 1992 · Separation Science and Technology; (United States)

DOI:https://doi.org/10.1080/01496399208019731· OSTI ID:5261544

De Bokx, P K; Baarslag, P C; Urbach, H P ^[1]

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1. Philips Research Labs., Eindhoven (Netherlands)

The chromatographic transport problem is a kinematic wave problem that generally leads to a set of coupled, nonlinear partial differential equations. In the linear elution mode of chromatography an approximation can be made which allows linearization of the set of equations and hence solution by the matrix eigenvalue decomposition method. To make the solution explicit, an expression for a multicomponent distribution isotherm is required. Generally, Langmuir isotherms are used for this purpose. In this work a recently published multicomponent isotherm that can be derived from first principles, is applied to the problem. For the case of binary eluents the capacity factors and peak compositions of both system peaks and analyte peaks are calculated using the elution mode approximation and the isotherm referred to above. Experimental results pertain to the ion-exchange separation of alkali ions. A good agreement between calculated and measured quantities is observed. An important consequence of interactions among sample and eluent constituents for the practice of chromatography lies in the field of indirect detection. The possibility of universal detection in LC is briefly discussed.

• OSTI ID: 5261544
• Journal Information: Separation Science and Technology; (United States), Journal Name: Separation Science and Technology; (United States) Vol. 27:7; ISSN SSTED; ISSN 0149-6395
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
ALKALI METALS
BINARY MIXTURES
CALCULATION METHODS
CHROMATOGRAPHY
DATA
DIFFERENTIAL EQUATIONS
DISPERSIONS
DISTRIBUTION
ELEMENTS
EQUATIONS
EXPERIMENTAL DATA
INFORMATION
ION EXCHANGE
ION EXCHANGE CHROMATOGRAPHY
KINETICS
METALS
MIXTURES
NUMERICAL DATA
PARTIAL DIFFERENTIAL EQUATIONS
SEPARATION PROCESSES