Tritium labeling of gonadotropin releasing hormone in its proline and histidine residues

Journal Article · · Peptides (Fayetteville, N.Y.); (United States)

DOI:https://doi.org/10.1016/S0196-9781(81)80103-9· OSTI ID:5260519

Klauschenz, E; Bienert, M; Egler, H; Pleiss, U; Niedrich, H; Nikolics, K

3,4-dehydroproline9-GnRH prepared by solid phase peptide synthesis was tritiated catalytically under various conditions yielding 3H-GnRH with specific radioactivities in the range from 35-60 Ci/mmol and full LH releasing activity in vitro. Using palladium/alumina catalyst, the tritiation of the double bond occurs within ten minutes. Investigation of the tritium distribution between the amino acid residues showed a remarkably high incorporation of tritium into the histidine residue (11 to 37%). On the basis of this observation, the tritium labeling of GnRH and angiotensin I by direct catalytic hydrogen-tritium exchange was found to be useful for the labeling of these peptides at remarkably high specific radioactivity.

• Research Organization: Institute of Drug Research, GDR Academy of Sciences, Berlin
• OSTI ID: 5260519
• Journal Information: Peptides (Fayetteville, N.Y.); (United States), Journal Name: Peptides (Fayetteville, N.Y.); (United States) Vol. 2:4; ISSN PPTDD
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
ALUMINIUM COMPOUNDS
ALUMINIUM OXIDES
AMINES
AMINO ACIDS
AZOLES
CARBOXYLIC ACIDS
CHALCOGENIDES
CHEMICAL PREPARATION
DRUGS
GONADOTROPINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
HORMONES
IMIDAZOLES
LABELLED COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PROLINE
PYRROLES
PYRROLIDINES
RADIOPHARMACEUTICALS
SYNTHESIS
TRITIUM COMPOUNDS