Radioactive labeling of $alpha$-bungarotoxin with tritium
Journal Article
·
· Anal. Biochem., v. 68, no. 2, pp. 394-403
OSTI ID:4124845
$alpha$-[$sup 3$H] bungarotoxin (Bgt) was prepared by catalytic reduction of $sup 125$I-labeled $alpha$-Bgt with tritium. Specific activities of 10 to 15 Ci/mmol were attained. The radioactive label was found in tyrosine. Tritiated $alpha$-Bgt appears to bind specifically to the cholinergic receptor of diaphragm and to a similar component of cerebral cortex. This specificity and the high specific radioactivity attained provide a useful tool for the study of acetylcholine receptor in brain and other tissues with low receptor concentration. (auth)
- Research Organization:
- Univ. of California, Berkeley
- NSA Number:
- NSA-33-009719
- OSTI ID:
- 4124845
- Journal Information:
- Anal. Biochem., v. 68, no. 2, pp. 394-403, Journal Name: Anal. Biochem., v. 68, no. 2, pp. 394-403; ISSN ANBCA
- Country of Publication:
- United States
- Language:
- English
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