Basolateral membrane of rat enterocyte: its purification from brush border contamination

Journal Article · Fri Feb 01 04:00:00 EST 1985 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(85)90156-3· OSTI ID:5247105

Orsenigo, M N; Tosco, M; Esposito, G; Faelli, A

Basolateral membranes obtained by self-orienting Percoll-gradient centrifugation were treated with 5 mM CaCl/sub 2/ to minimize the cross-contamination by brush border membranes. From marker enzyme-specific activities it was calculated that in this preparation the basolateral/brush border membrane ratio was 22.6. A low L-glucose permeability across basolateral membrane vesicles together with ATP-dependent sodium uptake was observed.

• Research Organization: Universita di Milano, Italy
• OSTI ID: 5247105
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 2; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
CALCIUM CHLORIDES
CALCIUM COMPOUNDS
CALCIUM HALIDES
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CENTRIFUGATION
CHLORIDES
CHLORINE COMPOUNDS
CYTOLOGICAL TECHNIQUES
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
GLUCOSE
HALIDES
HALOGEN COMPOUNDS
HEXOSES
INTESTINES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANES
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PERMEABILITY
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SODIUM 22
SODIUM COMPOUNDS
SODIUM ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES