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Cimetidine transport in rat renal brush border and basolateral membrane vesicles

Journal Article · · Life Sci.; (United States)
; ; ;
The transport of cimetidine by rat renal brush border and basolateral membrane vesicles has been studied in relation to the transport system of organic cation. Cimetidine inhibited (/sup 3/H)tetraethylammonium uptake by basolateral membrane vesicles in a dose dependent manner, and the degree of the inhibition was almost the same as that by unlabeled tetraethylammonium. In contrast, cimetidine inhibited the active transport of (/sup 3/H)tetraethylammonium by brush border membrane vesicles more strongly than unlabeled tetraethylammonium did. In agreement with the transport mechanism of tetraethylammonium in brush border membranes, the presence of an H/sup +/ gradient ((H/sup +/) i > (H/sup +/) o) induced a marked stimulation of cimetidine uptake against its concentration gradient (overshoot phenomenon), and this concentrative uptake was inhibited by unlabeled tetraethylammonium. These results suggest that cimetidine can share common carrier transport systems with tetraethylammonium in renal brush border and basolateral membranes, and that cimetidine transport across brush border membranes is driven by an H/sup +/ gradient via an H/sup +/-organic cation antiport system. 21 references, 3 figures, 2 tables.
Research Organization:
Kyoto Univ., Japan
OSTI ID:
5020995
Journal Information:
Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 37:17; ISSN LIFSA
Country of Publication:
United States
Language:
English

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Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BODY
CARBONIC ACID DERIVATIVES
CATIONS
CELL CONSTITUENTS
CELL MEMBRANES
CHARGED PARTICLES
DATA
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
GUANIDINES
HYDROGEN COMPOUNDS
INFORMATION
IONS
ISOTOPE APPLICATIONS
KIDNEYS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RATS
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES