Molecular cloning and sequence of the B880 holochrome gene from Rhodospirillum rubrum

Journal Article · Sun Jan 05 04:00:00 EST 1986 · J. Biol. Chem.; (United States)

OSTI ID:5246114

Restriction fragments of genomic Rhodospirillum rubrum DNA were selected according to size by electrophoresis followed by hybridization with (/sup 32/P)mRNA encoding the two B880 holochrome polypeptides. The fragments were cloned into Escherchia coli C600 with plasmid pBR327 as a vector. The clones were selected by colony hybridization with /sup 32/P-holochrome-mRNA and counter selected by hybridization with Rs. rubrum ribosomal RNA, a minor contaminant of the mRNA preparation. Chimeric plasmid pRR22 was shown to contain the B880 genes by hybrid selection of B880 holochrome-mRNA. A restriction map of its 2.2-kilobase insert and the sequence of a 430 base pair fragment thereof is reported. Genes ..cap alpha.. and ..beta.. are nearly contiguous, indicating that they are transcribed as a single operon. The predicted amino acid sequences coincide with the sequences of the ..cap alpha.. and ..beta.. polypeptides established in other laboratories, except for additional C-terminal tails of 10 and 13 amino acid residues, respectively.

• Research Organization: Universite de Montreal, Quebec
• OSTI ID: 5246114
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 261:1; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CLONING
DATA
DAYS LIVING RADIOISOTOPES
DNA
DNA-CLONING
ELECTROPHORESIS
ESCHERICHIA COLI
EXPERIMENTAL DATA
GENE OPERONS
GENETIC MAPPING
HYBRIDIZATION
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAPPING
MESSENGER-RNA
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RHODOSPIRILLUM
RIBOSOMES
RNA
TRACER TECHNIQUES
TRANSCRIPTION