Characterization of hemopoietic stem cell chimerism in antibody-facilitated bone marrow chimeras
The authors have previously described a model for bone marrow transplantation that involves preparation of the host with monoclonal antibody against class I or class II antigens instead of irradiation or cytotoxic drugs. This allows engraftment and subsequent repopulation of the host by donor tissue. They have previously reported on chimerism in the peripheral blood of P1----(P1 X P2)F1 animals. In this report, the authors describe the examination of the bone marrow and spleen stem cell chimerism of these antibody-facilitated (AF) chimeras, by determining, with an isozyme assay, the phenotype of methylcellulose colonies grown from stem cells. They have found a correlation between peripheral blood chimerism and the stem cell constitution of both spleen and bone marrow. The peripheral blood chimerism also correlates with the level of chimerism in macrophages derived from peritoneal exudate cells. These findings indicate that assaying the peripheral blood of such chimeras provides an excellent indication of the degree of chimerism at the stem cell level and stands in sharp contrast to the level of chimerism in certain lymphoid compartments.
- Research Organization:
- Univ. of Alberta, Edmonton, Canada
- OSTI ID:
- 5243839
- Journal Information:
- Transplantation; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHIMERAS
COMPARATIVE EVALUATIONS
STEM CELLS
GRAFT-HOST REACTION
ANTIGENS
BIOLOGICAL MODELS
BLOOD
BONE MARROW
HEMATOPOIETIC SYSTEM
MACROPHAGES
MICE
MONOCLONAL ANTIBODIES
SPLEEN
TRANSPLANTS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
MAMMALS
MATERIALS
MOSAICISM
ORGANS
PHAGOCYTES
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques
550901 - Pathology- Tracer Techniques