Effects of atrial natriuretic peptide, angiotensin, cyclic AMP, and potassium on protein phosphorylation in adrenal glomerulosa cells

Journal Article · Mon Dec 07 00:00:00 EST 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90436-X· OSTI ID:5243331

Elliott, M E; Goodfriend, T L

Bovine adrenal glomerulosa cells were incubated with /sup 32/PO/sub 4/ and angiotensin II (AII), atrial natriuretic peptide (ANP) (rat(8-33)), N/sup 6/,O/sup 2'/-dibutyryl cyclic AMP, or elevated potassium. Solubilized cells were analyzed by one-dimensional polyacrylamide gel electrophoresis, autoradiography, and laser densitometry. AII and dibutyryl cyclic AMO increased labeling of 17.6 kd protein. Elevated potassium did not alter labeling of this protein. ANP inhibited labeling, whether basal or stimulated by AII, and to a lesser extent that stimulated by dibutyryl cyclic AMP. Similar dose-response curves were obtained for the effect of AII on labeling of the 17.6 Kd band and on aldosterone synthesis; ANP had a similar inhibitory effect on AII-stimulated phosphorylation and aldosterone synthesis. Effects of AII and ANP were apparent after 15 minutes of hormone treatment. Fractionation of labeled cells showed that the 17.6 Kd protein was not in cytosol, mitochondria, or endoplasmic reticulum, but was enriched in a crude nuclear fraction. These results suggest AII and ANP affect aldosterone synthesis at the level of protein phosphorylation. 30 references, 5 figures, 1 table

• Research Organization: William S. Middleton Memorial Veterans Hospital, Madison, WI (USA)
• OSTI ID: 5243331
• Journal Information: Life Sci.; (United States), Vol. 41:23
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMP
BIOLOGICAL EFFECTS
ANGIOTENSIN
PEPTIDES
POTASSIUM
PROTEINS
PHOSPHORYLATION
ANIMAL CELLS
CATTLE
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
INHIBITION
KIDNEYS
PHOSPHORUS 32
TRACER TECHNIQUES
ALKALI METALS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR AGENTS
CHEMICAL REACTIONS
DATA
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
ELEMENTS
GLOBULINS
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
METALS
NUCLEI
NUCLEOTIDES
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RUMINANTS
VASOCONSTRICTORS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques