Effects of atrial natriuretic peptide, angiotensin, cyclic AMP, and potassium on protein phosphorylation in adrenal glomerulosa cells

Journal Article · Mon Dec 07 04:00:00 EST 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90436-X· OSTI ID:5243331

Elliott, M E; Goodfriend, T L

Bovine adrenal glomerulosa cells were incubated with /sup 32/PO/sub 4/ and angiotensin II (AII), atrial natriuretic peptide (ANP) (rat(8-33)), N/sup 6/,O/sup 2'/-dibutyryl cyclic AMP, or elevated potassium. Solubilized cells were analyzed by one-dimensional polyacrylamide gel electrophoresis, autoradiography, and laser densitometry. AII and dibutyryl cyclic AMO increased labeling of 17.6 kd protein. Elevated potassium did not alter labeling of this protein. ANP inhibited labeling, whether basal or stimulated by AII, and to a lesser extent that stimulated by dibutyryl cyclic AMP. Similar dose-response curves were obtained for the effect of AII on labeling of the 17.6 Kd band and on aldosterone synthesis; ANP had a similar inhibitory effect on AII-stimulated phosphorylation and aldosterone synthesis. Effects of AII and ANP were apparent after 15 minutes of hormone treatment. Fractionation of labeled cells showed that the 17.6 Kd protein was not in cytosol, mitochondria, or endoplasmic reticulum, but was enriched in a crude nuclear fraction. These results suggest AII and ANP affect aldosterone synthesis at the level of protein phosphorylation. 30 references, 5 figures, 1 table

• Research Organization: William S. Middleton Memorial Veterans Hospital, Madison, WI (USA)
• OSTI ID: 5243331
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 41:23; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METALS
AMP
ANGIOTENSIN
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARDIOVASCULAR AGENTS
CATTLE
CHEMICAL REACTIONS
DATA
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELEMENTS
EXPERIMENTAL DATA
GLOBULINS
INFORMATION
INHIBITION
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LIGHT NUCLEI
MAMMALS
METALS
NUCLEI
NUCLEOTIDES
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORYLATION
POTASSIUM
PROTEINS
RADIOISOTOPES
RUMINANTS
TRACER TECHNIQUES
VASOCONSTRICTORS
VERTEBRATES