Characterization of an alcohol dehydrogenase from Thermoanaerobacter ethanolicus active with ethanol and secondary alcohols
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
Thermoanaerobacter ethanolicus contains a NADP-dependent alcohol dehydrogenase. Ethanol is a substrate but secondary alcohols such as 2-propanol, 2-butanol and 2-pentanol are oxidized at a faster rate. The enzyme has a molecular weight of 172,000 and consists of 4 subunits. Each subunit contains 4 zinc atoms. With ethanol the kinetics are sigmoidal; however, in the presence of pyruvate a Michaelis-Menten kinetic pattern is approached. At 70/sup 0/C and pH 9 the purified enzyme oxidizes ethanol with NADP at a rate of 13 ..mu..mol min/sup -1/ mg/sup -1/, whereas it reduces acetaldehyde with NADPH two times that rate.
- Research Organization:
- Univ. of Georgia, Athens
- DOE Contract Number:
- AS09-79ER10499
- OSTI ID:
- 5242886
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 100:2; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
550500 -- Metabolism
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AEROBACTER
ALCOHOLS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CHEMICAL REACTIONS
COENZYMES
ELEMENTS
ENZYME ACTIVITY
ENZYMES
ETHANOL
HYDROXY COMPOUNDS
KINETICS
METABOLISM
METALS
MICROORGANISMS
MOLECULAR WEIGHT
NADP
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDATION
OXIDOREDUCTASES
REACTION KINETICS
SYNTHESIS
TEMPERATURE DEPENDENCE
ZINC
550500 -- Metabolism
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AEROBACTER
ALCOHOLS
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CHEMICAL REACTIONS
COENZYMES
ELEMENTS
ENZYME ACTIVITY
ENZYMES
ETHANOL
HYDROXY COMPOUNDS
KINETICS
METABOLISM
METALS
MICROORGANISMS
MOLECULAR WEIGHT
NADP
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDATION
OXIDOREDUCTASES
REACTION KINETICS
SYNTHESIS
TEMPERATURE DEPENDENCE
ZINC