Complete cDNA and derived amino acid sequence of human factor V
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
cDNA clones encoding human factor V have been isolated from an oligo(dT)-primed human fetal liver cDNA library prepared with vector Charon 21A. The cDNA sequence of factor V from three overlapping clones includes a 6672-base-pair (bp) coding region, a 90-bp 5' untranslated region, and a 163-bp 3' untranslated region within which is a poly(A)tail. The deduced amino acid sequence consists of 2224 amino acids inclusive of a 28-amino acid leader peptide. Direct comparison with human factor VIII reveals considerable homology between proteins in amino acid sequence and domain structure: a triplicated A domain and duplicated C domain show approx. 40% identity with the corresponding domains in factor VIII. As in factor VIII, the A domains of factor V share approx. 40% amino acid-sequence homology with the three highly conserved domains in ceruloplasmin. The B domain of factor V contains 35 tandem and approx. 9 additional semiconserved repeats of nine amino acids of the form Asp-Leu-Ser-Gln-Thr-Thr/Asn-Leu-Ser-Pro and 2 additional semiconserved repeats of 17 amino acids. Factor V contains 37 potential N-linked glycosylation sites, 25 of which are in the B domain, and a total of 19 cysteine residues.
- Research Organization:
- Univ. of Vermont, Burlington (USA)
- OSTI ID:
- 5242438
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:14; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Cloning of cDNAs coding for the heavy chain region and connecting region of human factor V, a blood coagulation factor with four types of internal repeats
Molecular cloning of the cDNA for human trkC (NTRK3), chromosomal assignment, and evidence for a splice variant
The mouse DNA binding protein Rc for the kappa B motif of transcription and for the V(D)J recombination signal sequences contains composite DNA-protein interaction domains and belongs to a new family of large transcriptional proteins
Journal Article
·
Tue Oct 06 00:00:00 EDT 1987
· Biochemistry; (United States)
·
OSTI ID:5640136
Molecular cloning of the cDNA for human trkC (NTRK3), chromosomal assignment, and evidence for a splice variant
Journal Article
·
Fri Jul 15 00:00:00 EDT 1994
· Genomics
·
OSTI ID:249982
The mouse DNA binding protein Rc for the kappa B motif of transcription and for the V(D)J recombination signal sequences contains composite DNA-protein interaction domains and belongs to a new family of large transcriptional proteins
Journal Article
·
Thu Aug 01 00:00:00 EDT 1996
· Genomics
·
OSTI ID:465975
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EVOLUTION
BLOOD COAGULATION FACTORS
BODY
CARBOHYDRATES
COAGULANTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA SEQUENCING
DRUGS
GENES
GLANDS
GLUCOPROTEINS
HEMATOLOGIC AGENTS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
LIVER
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EVOLUTION
BLOOD COAGULATION FACTORS
BODY
CARBOHYDRATES
COAGULANTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
DNA SEQUENCING
DRUGS
GENES
GLANDS
GLUCOPROTEINS
HEMATOLOGIC AGENTS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
LIVER
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS