Molecular cloning of the cDNA for human trkC (NTRK3), chromosomal assignment, and evidence for a splice variant

Journal Article · Fri Jul 15 04:00:00 EDT 1994 · Genomics

DOI:https://doi.org/10.1006/geno.1994.1383· OSTI ID:249982

McGregor, L M; Baylin, S B; Griffin, C A ^[1]

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1. Johns Hopkins Medical Institutions, Baltimore, MD (United States); and others

TrkC is a receptor tyrosine kinase that is activated by neurotrophin-3, a factor important in the development of certain areas of the central nervous system. The authors have cloned and sequenced the human trkC cDNA and found that the predicted amino acid sequence is 97 to 98% homologous to the rat and porcine trkC sequences, respectively. The rat trkC has several isoforms due to alternative splicing in the tyrosine kinase domain. They cloned one human splice variant that has a nucleic acid sequence identical to the rat isoform with an insert of 14 amino acids. The human trkC cDNA also has a (CGG){sub n} repeat in the 5{prime}-untranslated region. This sequence was not highly polymorphic in that 79 of 80 chromosomes examined had eight repeats, while 1 chromosome had four repeats. By PCR analysis of a somatic cell hybrid panel and fluorescence in situ hybridization with the cDNA clone, human trkC was mapped to chromosome 15q24-q25. 33 refs., 3 figs., 1 tab.

• OSTI ID: 249982
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 22; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GENES
GENETIC MAPPING
HUMAN CHROMOSOME 15
PHOSPHOTRANSFERASES
POLYMERASE CHAIN REACTION
RECEPTORS