Effects of zinc and PGE sub 2 on bone metabolism in chick tibia

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5238762

Chen, D; Pierce, Jr, W M ^[1]

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1. Univ. of Louisville, KY (United States)

The effects of zinc and prostaglandin E{sub 2} (PGE{sub 2}) on bone metabolism were investigated. Tibiae were removed from 19 day chicks and cultured for periods up to 72 hours in a chemically defined medium. Calcium release from tibiae cultured in the presence of 50-100 ug/dl zinc or 10{sup {minus}9}-10{sup {minus}7} M PGE{sub 2} between 24 and 48 hours increased significantly in a concentration dependent fashion. When 100 ug/dl zinc was added with 10{sup {minus}9}-10{sup {minus}7} M PGE{sub 2} to the culture bone, no additive effect was observed. Flurbiprofen decreased calcium release from chick tibiae significantly in the 24-48 hour incubation period in a concentration dependent fashion, suggesting that endogenous PG's have stimulatory effect on bone calcium release. When 10{sup {minus}6} M flurbiprofen was added with 25-100 ug/dl zinc, the calcium release from tibiae was not changed significantly. Bone release from tibiae was not changed significantly. Bone alkaline phosphatase activity was elevated by 50-100 ug/dl zinc. Addition of PGE{sub 2} did not increase the zinc stimulated elevation of alkaline phosphatase activity. The authors propose the following: zinc stimulates bone cell proliferation; PGE{sub 2} stimulates calcium release from the limited number of osteoclasts in cultured embryonic tibiae; and most bone organ culture system are models of zinc deficiency, since incubation of physiological concentrations of zinc has significant effect.

• OSTI ID: 5238762
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METALS
ALKALINE PHOSPHATASE
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIRDS
BODY
BONE CELLS
CALCIUM
CELL PROLIFERATION
CHICKENS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
ELEMENTS
ENZYME ACTIVITY
ENZYMES
ESTERASES
FOWL
HYDROLASES
METABOLISM
METALS
ORGANIC COMPOUNDS
ORGANS
PHOSPHATASES
PROSTAGLANDINS
PROTEINS
SECRETION
SKELETON
SOMATIC CELLS
TIBIA
VERTEBRATES
ZINC