Short-term effects of depot Zn and high dietary Zn on Zn, Cu, and Fe metabolism in rats

Conference · Fri Mar 15 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5238445

Reeves, P G; Nelson, K L; Bobilya, D J ^[1]

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1. Dept. of Agriculture, Grand Forks, ND (United States)

It was suggested that high intestinal mucosa metallothionein (IMMT) binds Cu and inhibits its absorption. Five-week old rats were fed for 1 week a diet containing 42 mg Zn, 6 mg Cu, and 50 mg Fe/kg (N-ZN). Then they were divided into 3 groups. One group continued to receive the N-ZN while another group received a similar diet containing 560 mg Zn/kg (HiZN). A third group, fed the N-ZN diet, received a s.c. dose of 90 mg Zn/kg BW every 2-3 days (DpZN) for the duration of the experiment. Rats from each group were killed on days 7 and 14. After 7 days, serum Zn in the HiZN and DpZN groups was 3-times higher than in the N-ZN group. Serum Cu was 50% higher in DpZN than N-ZN rats, but it was 30% lower in HiZN and N-ZN rats. Fe in serum of both DpZN and HiZN rats was lower than N-ZN, but it was lowest in HiZN rats. After 14 days, serum Zn in the DpZN group was 3 1/2-times higher than the N-ZN group but in the serum of HiZN rats, Zn was only 1 1/2-times higher than N-ZN rats. Serum Cu in both DpZN and HiZN groups was < 1/2 that of the N-ZN group. At 7 days, IMMT was 15-times higher in both Zn-treated groups than in the N-ZN group. At day 14, IMMT had risen most in the DpZN group and was 32-times that of the N-ZN group. IMMT in the HiZN group had decreased from day 7, and was only 8-times higher than the N-ZN group. The concentration of Zn in the mucosa followed that of IMMT at both periods. By contrast, mucosal Cu at 7 days was significantly lower in the HiZN than either of the other two groups. N-ZN and DpZN were not different from each other. Mucosal Fe was not significantly affected by Zn treatment. At day 14, mucosal Cu in both Zn-treated groups was only 1/2 that of the N-ZN group. These data suggest that there is no consistent relationship between high IMMT and altered Cu metabolism in short-term Zn-treated rats.

• OSTI ID: 5238445
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:5
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism &amp; Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
COPPER
DIET
DIGESTIVE SYSTEM
ELEMENTS
GASTROINTESTINAL TRACT
INTESTINAL ABSORPTION
INTESTINES
IRON
KINETICS
MAMMALS
MEMBRANES
METABOLISM
METALLOPROTEINS
METALLOTHIONEIN
METALS
MUCOUS MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RATS
REACTION KINETICS
RODENTS
TRANSITION ELEMENTS
UPTAKE
VERTEBRATES
ZINC