Iron distances in hemoglobin: comparison of x-ray crystallographic and extended x-ray absorption fine structure studies

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.17.6167· OSTI ID:5235852

Fermi, G; Perutz, M F; Shulman, R G

A comparison is presented of the structures obtained around the iron atom in deoxyhemoglobin (Hb). The data come from extended x-ray absorption fine structure (EXAFS) studies of the iron, which gave Fe-porphyrin nitrogen distances of 2.06 +- 0.01 A, and from the most recent high-resolution x-ray crystallographic study, which gave exactly the same distance-2.06 +- 0.02 A. The distance of Fe above the plane of the porphyrin nitrogens was 0.38 +- 0.04 A from the crystallographic study; this value is not far from the upper limit of the distances 0.20 +- /sub 0.20//sup 0.10/ A calculated from the EXAFS experiment by triangulation. These distances above the nitrogen plane are shorter than those estimated in the earliest x-ray structures

• Research Organization: Medical Research Council Lab. of Molecular Biology, Cambridge (England)
• OSTI ID: 5235852
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:17; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550602* -- Medicine-- External Radiation in Diagnostics-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
CARBOXYLIC ACIDS
COHERENT SCATTERING
CRYSTALLOGRAPHY
DIFFRACTION
ELEMENTS
GLOBIN
HEME
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IRON
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
SCATTERING
TRANSITION ELEMENTS
X-RAY DIFFRACTION