Dexamethasone regulates beta adrenergic receptors in 3T3-L1 fibroblasts

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5234290

Nakada, M T; Stadel, J M; Poksay, K S; Crooke, S T

3T3-L1 fibroblasts, containing ..beta..-adrenergic receptors (..beta..AR) of predominantly the ..beta../sub 1/AR subtype, express only ..beta../sub 2/AR after 48 hr treatment with 250 nM dexamethasone (dex). A two-fold increase in ..beta..AR number was also observed upon dex treatment. ..beta..AR were assayed in membranes using the radioligand (/sup 125/I)-cyanopindolol and the ..beta../sub 2/AR selective antagonist ICI. The relative potency of agonists in stimulating whole cell adenylate cyclase activity was consistent with the subtypes determined by binding experiments. The ability of compounds to cause the subtype switch correlates with glucocorticoid activity since these compounds were more effective in facilitating the subtype conversion and the increase in ..beta..AR number than mineralocorticoids. Sex steroids and thyroid hormone were ineffective at concentrations up to 1..mu..M in causing these changes. RNA and protein synthesis appear to be required for the ..beta..AR subtype switch and increase in ..beta..AR number since these changes were not observed with dex treatment in the presence of 1..mu..g/ml actinomycin D or 1..mu..g/ml cyclohexamide. This study suggests that glucocorticoids may induce gene activation in 3T3-L1 fibroblasts that results in an increase in ..beta..AR number and a shift in subtype.

• Research Organization: SmithKline and French Labs., Philadelphia, PA
• OSTI ID: 5234290
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
AMINO ACIDS
ANIMAL CELLS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
CYCLASES
DAYS LIVING RADIOISOTOPES
DEXAMETHASONE
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
FIBROBLASTS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
LIGANDS
LYASES
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PEPTIDE HORMONES
PREGNANES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RNA
SOMATIC CELLS
STEROID HORMONES
STEROIDS
SYMPATHOMIMETICS
THYROID HORMONES
THYROXINE
TRACER TECHNIQUES