Specific interaction of aurintricarboxylic acid with the human immunodeficiency virus/CD4 cell receptor

Journal Article · Mon May 01 04:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.9.3322· OSTI ID:5228429

Schols, D; Baba, M; Pauwels, R; Desmyter, J; De Clercq, E ^[1]

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1. Katholieke Universiteit Leuven (Belgium)

The triphenylmethane derivative aurintricarboxylic acid (ATA), but not aurin, selectively prevented the binding of OKT4A/Leu-3a monoclonal antibody (mAb) and, to a lesser extent, OKT4 mAb to the CD4 cell receptor for human immunodeficiency virus type 1 (HIV-1). The effect was seen within 1 min at an ATA concentration of 10 {mu}M in various T4{sup +} cells (MT-4, U-937, peripheral blood lymphocytes, and monocytes). It was dose-dependent and reversible. ATA prevented the attachment of radiolabeled HIV-1 particles to MT-4 cells, which could be expected as the result of its specific binding to the HIV/CD4 receptor. Other HIV inhibitors such as suramin, fuchsin acid, azidothymidine, dextran sulfate, heparin, and pentosan polysulfate did not affect OKT4A/Leu-3a mAb binding to the CD4 receptor, although the sulfated polysaccharides suppressed HIV-1 adsorption to the cells at concentrations required for complete protection against HIV-1 cytopathogenicity. Thus, ATA is a selective marker molecule for the CD4 receptor. ATA also interfered with the staining of membrane-associated HIV-1 glycoprotein gp120 by a mAb against it. These unusual properties of a small molecule of nonimmunological origin may have important implications for the study of CD4/HIV/AIDS pathogenesis and possibly treatment.

• OSTI ID: 5228429
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:9; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AIDS VIRUS
ALUMINON
ANIMAL CELLS
ANTIBODIES
AZINES
BIOLOGICAL MARKERS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
CROSS-LINKING
DYES
FLUORESCENCE
GLYCOPROTEINS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
HYDROXY COMPOUNDS
LEUKOCYTES
LUMINESCENCE
LYMPHOCYTES
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MICROORGANISMS
MOLECULAR BIOLOGY
MONOCLONAL ANTIBODIES
MONOCYTES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASITES
POLYMERIZATION
PROTEINS
PYRIMIDINES
REAGENTS
RECEPTORS
RIBOSIDES
SOMATIC CELLS
TRIPHENYLMETHANE DYES
TRITIUM COMPOUNDS
URACILS
URIDINE
VIRUSES