Misonidazole binding in murine liver tissue: A marker for cellular hypoxia in vivo
Journal Article
·
· Gastroenterology; (USA)
OSTI ID:5227969
- Queen's Univ. of Belfast (Northern Ireland)
The hepatic microcirculation is believed to cause variable cellular oxygenation within the organ. In this study a marker of cellular hypoxia was used to demonstrate liver oxygen tension gradients in vivo. Covalent binding of misonidazole adducts to cellular macromolecules is enhanced by hypoxia. Autoradiographs of liver from mice treated with radiolabeled misonidazole demonstrated enhanced binding of adducts within hepatocytes surrounding hepatic veins. Livers from both hypoxic and normal mice had characteristic autoradiographic grain patterns reflecting regional oxygen tension variation in vivo. Differential binding of misonidazole adducts formed in hypoxic cells could have an application in studies of liver physiology and biochemistry.
- OSTI ID:
- 5227969
- Journal Information:
- Gastroenterology; (USA), Journal Name: Gastroenterology; (USA) Vol. 97:5; ISSN GASTA; ISSN 0016-5085
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANOXIA
ANTINEOPLASTIC DRUGS
AUTORADIOGRAPHY
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MARKERS
BODY
CARBON ISOTOPES
DIGESTIVE SYSTEM
DRUGS
ELEMENTS
GLANDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
ISOTOPES
KINETICS
LIVER
MAMMALS
MICE
MISONIDAZOLE
NONMETALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXYGEN
RADIOSENSITIZERS
REACTION KINETICS
RODENTS
VERTEBRATES
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANOXIA
ANTINEOPLASTIC DRUGS
AUTORADIOGRAPHY
AZOLES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MARKERS
BODY
CARBON ISOTOPES
DIGESTIVE SYSTEM
DRUGS
ELEMENTS
GLANDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
ISOTOPES
KINETICS
LIVER
MAMMALS
MICE
MISONIDAZOLE
NONMETALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXYGEN
RADIOSENSITIZERS
REACTION KINETICS
RODENTS
VERTEBRATES