Specific killing of human melanoma cells with an efficient 10B-compound on monoclonal antibodies

Journal Article · Sat Jul 01 04:00:00 EDT 1989 · Pigment Cell Research; (USA)

DOI:https://doi.org/10.1111/j.1600-0749.1989.tb00201.x· OSTI ID:5225621

Komura, A; Tokuhisa, T; Nakagawa, T; Sasase, A; Ichihashi, M; Ferrone, S; Mishima, Y ^[1]

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1. Kobe Univ. (Japan)

We previously established methods which have enabled us to target a sufficient number of 10B atoms on human melanoma cells to destroy them by thermal neutron irradiation. Monoclonal antibodies were here used as vector of 10B atoms on the target cell. Thermal neutrons require at least 10(9) 10B atoms to destroy the cell. In order to accumulate an adequate number of 10B atoms on target cells, our first approach was to make an effective compound that contains 12 atoms of 10B in a molecule. The second step was to conjugate the compound with an avidin molecule (10B12-avidin). One molecule of the 10B12-avidin carries about 30 atoms of 10B. This 10B12-avidin can be specifically targeted on human melanoma cells by biotinated monoclonal antibodies specific for the cells. Furthermore, the number of 10B atoms on target cells can be augmented by a hapten-antihapten monoclonal antibody system. The cultured human melanoma cells treated with these methods were damaged by thermal neutron irradiation. This is the first study that indicates thermal neutrons do injure target cells boronated by monoclonal antibodies.

• OSTI ID: 5225621
• Journal Information: Pigment Cell Research; (USA), Journal Name: Pigment Cell Research; (USA) Vol. 2:4; ISSN 0893-5785; ISSN PCREE
• Country of Publication: United States
• Language: English

Related Subjects

550604* -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMALS
ANTIBODIES
BEAMS
BORON 10
BORON COMPOUNDS
BORON ISOTOPES
CELL KILLING
DISEASES
IMMUNOLOGY
IMMUNOTHERAPY
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MEDICINE
MELANOMAS
MONOCLONAL ANTIBODIES
NEOPLASMS
NEUTRON BEAMS
NEUTRON CAPTURE THERAPY
NEUTRON THERAPY
NUCLEAR MEDICINE
NUCLEI
NUCLEON BEAMS
ODD-ODD NUCLEI
PARTICLE BEAMS
PRIMATES
RADIOIMMUNOLOGY
RADIOIMMUNOTHERAPY
RADIOLOGY
RADIOTHERAPY
STABLE ISOTOPES
SURVIVAL TIME
THERAPY
TUMOR CELLS
VERTEBRATES