Failure to induce a DNA repair gene, RAD54, in Saccharomyces cerevisiae does not affect DNA repair or recombination phenotypes
- Univ. of California, Berkeley (USA)
The Saccharomyces cerevisiae RAD54 gene is transcriptionally regulated by a broad spectrum of DNA-damaging agents. Induction of RAD54 by DNA-damaging agents is under positive control. Sequences responsible for DNA damage induction (the DRS element) lie within a 29-base-pair region from -99 to -70 from the most proximal transcription start site. This inducible promoter element is functionally separable from a poly(dA-dT) region immediately downstream which is required for constitutive expression. Deletions which eliminate induction of RAD54 transcription by DNA damage but do not affect constitutive expression have no effect on growth or survival of noninducible strains relative to wild-type strains in the presence of DNA-damaging agents. The DRS element is also not required for homothallic mating type switching, transcriptional induction of RAD54 during meiosis, meiotic recombination, or spontaneous or X-ray-induced mitotic recombination. We find no phenotype for a lack of induction of RAD54 message via the damage-inducible DRS, which raises significant questions about the physiology of DNA damage induction in S. cerevisiae.
- OSTI ID:
- 5224784
- Journal Information:
- Molecular and Cellular Biology; (USA), Vol. 9:8; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
SACCHAROMYCES CEREVISIAE
DNA REPAIR
AMINO ACID SEQUENCE
GENE RECOMBINATION
GENE REGULATION
GENETIC RADIATION EFFECTS
PHENOTYPE
PLASMIDS
TRANSCRIPTION
X RADIATION
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
ELECTROMAGNETIC RADIATION
EUMYCOTA
FUNGI
GENETIC EFFECTS
IONIZING RADIATIONS
MICROORGANISMS
MOLECULAR STRUCTURE
PLANTS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SACCHAROMYCES
YEASTS
560130* - Radiation Effects on Microorganisms