Failure to induce a DNA repair gene, RAD54, in Saccharomyces cerevisiae does not affect DNA repair or recombination phenotypes

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.9.8.3314· OSTI ID:5224784

Cole, G M; Mortimer, R K ^[1]

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1. Univ. of California, Berkeley (USA)

The Saccharomyces cerevisiae RAD54 gene is transcriptionally regulated by a broad spectrum of DNA-damaging agents. Induction of RAD54 by DNA-damaging agents is under positive control. Sequences responsible for DNA damage induction (the DRS element) lie within a 29-base-pair region from -99 to -70 from the most proximal transcription start site. This inducible promoter element is functionally separable from a poly(dA-dT) region immediately downstream which is required for constitutive expression. Deletions which eliminate induction of RAD54 transcription by DNA damage but do not affect constitutive expression have no effect on growth or survival of noninducible strains relative to wild-type strains in the presence of DNA-damaging agents. The DRS element is also not required for homothallic mating type switching, transcriptional induction of RAD54 during meiosis, meiotic recombination, or spontaneous or X-ray-induced mitotic recombination. We find no phenotype for a lack of induction of RAD54 message via the damage-inducible DRS, which raises significant questions about the physiology of DNA damage induction in S. cerevisiae.

• OSTI ID: 5224784
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 9:8; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACID SEQUENCE
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
DNA REPAIR
ELECTROMAGNETIC RADIATION
EUMYCOTA
FUNGI
GENE RECOMBINATION
GENE REGULATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
MICROORGANISMS
MOLECULAR STRUCTURE
PHENOTYPE
PLANTS
PLASMIDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
TRANSCRIPTION
X RADIATION
YEASTS