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Synthesis, storage, and stability of (4-/sup 14/C)oxaloacetic acid

Journal Article · · Anal. Biochem.; (United States)

A simple procedure for preparing (4-/sup 14/C)oxaloacetic acid based on the reaction between (/sup 14/C)HCO-3 and phosphoenolpyruvate catalyzed by phosphoenolpyruvate carboxylase is described. A simple method for preparing highly purified phosphoenolpyruvate carboxylase from maize leaves is described and the degradation of oxaloacetate under conditions of varying pH and divalent metal ion concentration is reported. (4-/sup 14/C)Oxaloacetic acid is stable for several months in 0.1 M HCl solution at -80 degrees C.

Research Organization:
CSIRO, Canberra ACT, Australia
OSTI ID:
5220754
Journal Information:
Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Journal Issue: 2 Vol. 2; ISSN ANBCA
Country of Publication:
United States
Language:
English