L-(4-/sup 11/C)aspartic acid: enzymatic synthesis, myocardial uptake, and metabolism

Journal Article · Thu Dec 31 23:00:00 EST 1981 · J. Med. Chem.; (United States)

DOI:https://doi.org/10.1021/jm00343a020· OSTI ID:7070599

Barrio, J R; Egbert, J E; Henze, E; Schelbert, H R; Baumgartner, F J

Sterile, pyrogen-free L-(4-/sup 11/C)aspartic acid was prepared from /sup 11/CO/sub 2/ using phosphoenolpyruvate carboxylase and glutamic/oxaloacetic acid transaminase immobilized on Sepharose supports to determine if it is a useful indicator for in vivo, noninvasive determination of myocardial metabolism. An intracoronary bolus injection of L-(4-/sup 11/C)aspartic acid into dog myocardium showed a triexponential clearance curve with maximal production of /sup 11/CO/sub 2/ 100 s after injection. Inactivation of myocardial transaminase activity modified the tracer clearance and inhibited the production of /sup 11/CO/sub 2/. Positron-computed tomography imaging showed that the /sup 11/C activities retained in rhesus monkey myocardium are higher than those observed in dog heart after intravenous injection of L-(4-/sup 11/C)aspartic acid. These findings demonstrated the rapid incorporation of the carbon skeleton of L-aspartic acid into the tricarboxylic acid cycle after enzymatic transamination in myocardium and suggested that L-(4-/sup 11/C)aspartic acid could be of value for in vivo, noninvasive assessment of local myocardial metabolism.

• Research Organization: Department of Radiological Sciences, Laboratory of Nuclear Medicine, University of California at Los Angeles
• OSTI ID: 7070599
• Journal Information: J. Med. Chem.; (United States), Journal Name: J. Med. Chem.; (United States) Vol. 25:1; ISSN JMCMA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
AMINOTRANSFERASES
ANIMALS
ASPARTIC ACID
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
CARBON 11
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON ISOTOPES
CARBON OXIDES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHALCOGENIDES
CHEMICAL PREPARATION
CLEARANCE
COMPUTERIZED TOMOGRAPHY
COUNTING TECHNIQUES
DIAGNOSTIC TECHNIQUES
DIAGNOSTIC USES
DOGS
DRUGS
EMISSION COMPUTED TOMOGRAPHY
ENZYMES
EVEN-ODD NUCLEI
HEART
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MACACUS
MAMMALS
METABOLISM
MINUTES LIVING RADIOISOTOPES
MONKEYS
MUSCLES
MYOCARDIUM
NITROGEN TRANSFERASES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
POSITRON COMPUTED TOMOGRAPHY
PRIMATES
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOPHARMACEUTICALS
SCINTISCANNING
SYNTHESIS
TOMOGRAPHY
TRANSFERASES
USES
VERTEBRATES