Use of 3,4-dichlorobenzenethiol as a trapping agent for alkylating intermediates during in vitro metabolism of nitrosamines
Journal Article
·
· Cancer Res.; (United States)
OSTI ID:5220486
Studies using 3,4-dichlorobenzenethiol as a probe for methylating agent production during exposure of N-nitrosodimethylamine to rat liver S-9 preparations produced results different from those of an investigation reported in the literature. Methyl-3,4-dichlorophenyl thioether was detected, but the quantities found were not significantly different from the background levels of methylation product detected in the absence of nitrosamine. Only about 10% of the thioether isolated after incubating N-nitrosodi(/sup 14/C)methylamine as substrate was radioactive. The results indicate that the majority of the methyl groups transferred to the sulfur nucleophile in these experiments came from components of the incubation mixture other than the nitrosamine. Some artifactual methylation was also associated with the analytical procedure. The authors conclude that 3,4-dichlorobenzenethiol should be used with caution in studies of alkylation during the in vitro metabolism of carcinogenic nitrosamines.
- Research Organization:
- Frederick Cancer Research Facility, MD
- OSTI ID:
- 5220486
- Journal Information:
- Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 7; ISSN CNREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501* -- Metabolism-- Tracer Techniques
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMINES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CARBON 14 COMPOUNDS
CARCINOGEN SCREENING
CARCINOGENS
CHALCOGENIDES
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
GLANDS
IN VITRO
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
METHYLATION
NITROSAMINES
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RATS
REACTION KINETICS
RODENTS
SCREENING
SULFIDES
SULFUR COMPOUNDS
THIOLS
TRACER TECHNIQUES
USES
VERTEBRATES
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMINES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CARBON 14 COMPOUNDS
CARCINOGEN SCREENING
CARCINOGENS
CHALCOGENIDES
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
GLANDS
IN VITRO
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIVER
MAMMALS
METABOLISM
METHYLATION
NITROSAMINES
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RATS
REACTION KINETICS
RODENTS
SCREENING
SULFIDES
SULFUR COMPOUNDS
THIOLS
TRACER TECHNIQUES
USES
VERTEBRATES